HAI-1, His, Human

HAI-1, His, Human

SPINT1, also known as HAI-1, is a Kunitz-type serine protease inhibitor that inhibits multiple proteases including hepatocyte growth factor (HGF) activator and matriptase. SPINT1 has been shown to modulate HGF/MET activation in certain cancer types.
Z05370
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Product Introduction
Species Human
Protein Construction
HAI-1 (Gly36-Val433)
Accession # O43278-2		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca­ArgPro­Lys­Pro­Val­Glu­NVAL­Trp­Arg­Lys(DNP)­NH2. The IC50 is < 1 nM. Test result meets the standard.
Expression System HEK293
Theoretical Molecular Weight 45.50 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Background
Target Background SPINT1, also known as HAI-1, is a Kunitz-type serine protease inhibitor that inhibits multiple proteases including hepatocyte growth factor (HGF) activator and matriptase. SPINT1 has been shown to modulate HGF/MET activation in certain cancer types.
Synonyms HAI 1; SPINT1; HAI1; HAI-1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.