FGF basic, Human
FGF Basic, Human

Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 0.1 - 0.6 ng/ml (QC Test).

FGF Basic, Human

Immobilized FGF basic, Human, No Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 13.7ng/ml determined by ELISA.

FGF Basic, Human

Immobilized FGF basic, Human, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human FGFR2 beta (IIIc), His Tag with the EC50 of 1.49μg/ml determined by ELISA.

FGF Basic, Human

FGF basic, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

FGF basic, Human

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.
Z05288
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Product Introduction
Species Human
Protein Construction
FGF basic (Pro143-Ser288)
Accession # P09038-4
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 0.02 EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System E.coli
Theoretical Molecular Weight 16.5 kDa
Apparent Molecular Weight The protein has a predicted MW of 16.5 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • FGF Basic, Human
    • FGF Basic, Human

    Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 0.1 - 0.6 ng/ml (QC Test).

  • FGF Basic, Human
    • FGF Basic, Human

    Immobilized FGF basic, Human, No Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 13.7ng/ml determined by ELISA.

  • FGF Basic, Human
    • FGF Basic, Human

    Immobilized FGF basic, Human, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human FGFR2 beta (IIIc), His Tag with the EC50 of 1.49μg/ml determined by ELISA.

  • FGF Basic, Human
    • FGF Basic, Human

    FGF basic, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.
Synonyms bFGF; FGF basic; FGF2; FGF-2; FGF2AS; GFG1; HBGF-2; NUDT6; Prostatropin; BFGF; FGFB; FGFβ; HBGF2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.