ENPP-1, His, Human
ENPP-1, His, Human

Immobilized ENPP-1, His, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 22.1 ng/ml determined by ELISA.

ENPP-1, His, Human

ENPP-1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ENPP-1, His, Human

Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.
Z05239
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Product Introduction
Species Human
Protein Construction
His ENPP-1 (Lys98-Asp925)
Accession # P22413
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized ENPP-1, His, Human at 0.5μg/ml (100μl/well) on the plate can bind Anti­ENPP­1 Antibody, hFc Tag. Test result was comparable to standard batch. Measured by its ability to hydrolyze thymidine 5'­monophosphate p­nitrophenyl ester. The specific activity is >40000 pmol/min/μg. Test result meets the standard.
Expression System HEK293
Theoretical Molecular Weight 96.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 110-115 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • ENPP-1, His, Human
    • ENPP-1, His, Human

    Immobilized ENPP-1, His, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 22.1 ng/ml determined by ELISA.

  • ENPP-1, His, Human
    • ENPP-1, His, Human

    ENPP-1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.
Synonyms E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.