EGF, Human
EGF, Human

Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1 - 0.2 ng/mL (QC Test).

EGF, Human

Immobilized EGF, Human, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.59 μg/ml determined by ELISA.

EGF, Human

The purity of EGF, Human is greater than 95% as determined by SEC-HPLC.

EGF, Human

EGF, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

EGF, Human

Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind EGF, Human, No Tag with an affinity constant of 1.33 nM as determined in SPR assay (Biacore T200).

EGF, Human

The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
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Product Introduction
Species Human
Protein Construction
EGF (Asn971-Arg1023)
Accession # P01133-1
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 0.2EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System E.coli
Theoretical Molecular Weight 5.6 kDa
Apparent Molecular Weight The protein has a predicted MW of 5.6 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • EGF, Human
    • EGF, Human

    Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1 - 0.2 ng/mL (QC Test).

  • EGF, Human
    • EGF, Human

    Immobilized EGF, Human, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.59 μg/ml determined by ELISA.

  • EGF, Human
    • EGF, Human

    The purity of EGF, Human is greater than 95% as determined by SEC-HPLC.

  • EGF, Human
    • EGF, Human

    EGF, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • EGF, Human
    • EGF, Human

    Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind EGF, Human, No Tag with an affinity constant of 1.33 nM as determined in SPR assay (Biacore T200).


Background
Target Background The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Synonyms HOMG4; EGF; URG

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.