EGF, Human

EGF, Human

The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Z05234
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Product Introduction
Species Human
Protein Construction
EGF (Asn971-Arg1023)
Accession # P01133-1
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 0.2EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized EGF, Human at 2μg/ml (100μl/well) on the plate can bind Human EGFR, hFc Tag. Test result was comparable to standard batch.

Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1 ­ 0.2 ng/mL. Test result was comparable to standard batch.
Expression System E.coli
Theoretical Molecular Weight 5.6 kDa
Apparent Molecular Weight The protein has a predicted MW of 5.6 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Background
Target Background The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Synonyms HOMG4; EGF; URG

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.