EGF hFc Chimera, Human
EGF HFc Chimera, Human

Loaded EGF hFc Chimera, Human, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.25 µM as determined in BLI assay (Gator® Prime).

EGF HFc Chimera, Human

Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.2 - 1.5 ng/mL.

EGF HFc Chimera, Human

Immobilized Biotinylated Human EGFR, His-Avi Tag at 1 μg/ml (100 μI/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for EGF hFc Chimera, Human, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.

EGF HFc Chimera, Human

The purity of EGF hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

EGF HFc Chimera, Human

EGF hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

EGF HFc Chimera, Human

Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind EGF hFc Chimera, Human, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay (Biacore T200).

EGF hFc Chimera, Human

The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
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Product Introduction
Species Human
Protein Construction
hFc EGF (Asn971-Arg1023)
Accession # P01133-1
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 32.52 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 38-43 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • EGF HFc Chimera, Human
    • EGF HFc Chimera, Human

    Loaded EGF hFc Chimera, Human, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.25 µM as determined in BLI assay (Gator® Prime).

  • EGF HFc Chimera, Human
    • EGF HFc Chimera, Human

    Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.2 - 1.5 ng/mL.

  • EGF HFc Chimera, Human
    • EGF HFc Chimera, Human

    Immobilized Biotinylated Human EGFR, His-Avi Tag at 1 μg/ml (100 μI/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for EGF hFc Chimera, Human, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.

  • EGF HFc Chimera, Human
    • EGF HFc Chimera, Human

    The purity of EGF hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

  • EGF HFc Chimera, Human
    • EGF HFc Chimera, Human

    EGF hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • EGF HFc Chimera, Human
    • EGF HFc Chimera, Human

    Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind EGF hFc Chimera, Human, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay (Biacore T200).


Background
Target Background The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Synonyms HOMG4; EGF; URG

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.