EFEMP1, His, Human
EFEMP1, His, Human

Immobilized EFEMP1, His, Human, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 29.6 ng/ml determined by ELISA.

EFEMP1, His, Human

EFEMP1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

EFEMP1, His, Human

Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion.
Z05232
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Product Introduction
Species Human
Protein Construction
His EFEMP1 (Gln18-Phe493)
Accession # Q12805-1
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized EFEMP1, His, Human at 2μg/ml (100μl/Well) on the plate can bind Anti­EFEMP1 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 53.9 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • EFEMP1, His, Human
    • EFEMP1, His, Human

    Immobilized EFEMP1, His, Human, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 29.6 ng/ml determined by ELISA.

  • EFEMP1, His, Human
    • EFEMP1, His, Human

    EFEMP1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion.
Synonyms EFEMP1; FBLN3; FBNL; DHRD; FBNLFLJ35535; FIBL-3; fibrillin-like; Fibulin 3; MGC111353; MLVT; MTLV; S1-5

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.