DKK1, His, Human
DKK1, His, Human

Immobilized DKK1, His, Human, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.50 μg/ml determined by ELISA.

DKK1, His, Human

DKK1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

DKK1, His, Human

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Z05219
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Product Introduction
Species Human
Protein Construction
DKK1 (Thr32-His266)
Accession # O94907		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized DKK1, His, Human at 5μg/ml (100μl/well) on the plate can bind Human LRP­5, mFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 26.87 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • DKK1, His, Human
    • DKK1, His, Human

    Immobilized DKK1, His, Human, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.50 μg/ml determined by ELISA.

  • DKK1, His, Human
    • DKK1, His, Human

    DKK1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Synonyms Dickkopf-1; Dkk1; SK

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.