DKK1 C terminal Domain hFc Chimera, Avi, Human
DKK1 C Terminal Domain HFc Chimera, Avi, Human

Immobilized DKK1 C terminal Domain hFc Chimera, Avi, Human, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-DKK1 Antibody, hFc Tag with the EC50 of 29.5 ng/ml determined by ELISA.

DKK1 C Terminal Domain HFc Chimera, Avi, Human

DKK1 C terminal Domain hFc Chimera, Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

DKK1 C terminal Domain hFc Chimera, Avi, Human

Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
Z05215
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Product Introduction
Species Human
Protein Construction
DKK1 C terminal Domain (Met178-His266)
Accession # O94907		 hFc Avi
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized DKK1 C terminal Domain hFc Chimera, Avi, Human at 0.5μg/ml (100μl/Well) on the plate can bind Biotinylated Anti­DKK1 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 38.69 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in 20mM NaAc,150mM NaCl (pH 5.0).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0).
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • DKK1 C Terminal Domain HFc Chimera, Avi, Human
    • DKK1 C Terminal Domain HFc Chimera, Avi, Human

    Immobilized DKK1 C terminal Domain hFc Chimera, Avi, Human, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-DKK1 Antibody, hFc Tag with the EC50 of 29.5 ng/ml determined by ELISA.

  • DKK1 C Terminal Domain HFc Chimera, Avi, Human
    • DKK1 C Terminal Domain HFc Chimera, Avi, Human

    DKK1 C terminal Domain hFc Chimera, Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
Synonyms Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.