Loaded Anti-Coagulation factor III Antibody, hFc Tag on Protein A-Biosensor, can bind Coagulation Factor III/Tissue Factor, His, Human, His Tag with an affinity constant of 5.57 nM as determined in BLI assay (Gator).

Immobilized Coagulation Factor III/Tissue Factor, His, Human, His Tag at 2 μg /ml (100 μl /well) on the plate. Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.

The purity of Coagulation Factor III/Tissue Factor, His, Human is greater than 95% as determined by SEC-HPLC.

Coagulation Factor III/Tissue Factor, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Anti-Coagulation factor III Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Coagulation Factor III/Tissue Factor, His, Human, His Tag with an affinity constant of 9.25 nM as determined in SPR assay (Biacore T200).

Coagulation Factor III/Tissue Factor, His, Human

Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.

Cat. No.	Z05184
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
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Price	¥63,425.00


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Product Introduction

Species

Human

Protein Construction

Coagulation factor III/Tissue Factor (Ser33-Glu251) Accession # P13726-1	His
N-term	C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

25.89 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

The purity of Coagulation Factor III/Tissue Factor, His, Human is greater than 95% as determined by SEC-HPLC.

Coagulation Factor III/Tissue Factor, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Background

Target Background	Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.
Synonyms	Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Coagulation Factor III/Tissue Factor, His, Human

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