B7-H2/ICOSLG, His & Avi, Human
B7-H2/ICOSLG, His & Avi, Human

Immobilized B7-H2/ICOSLG, His & Avi, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA.

B7-H2/ICOSLG, His & Avi, Human

The purity of B7-H2/ICOSLG, His & Avi, Human is greater than 90% as determined by SEC-HPLC.

B7-H2/ICOSLG, His & Avi, Human

B7-H2/ICOSLG, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

B7-H2/ICOSLG, His & Avi, Human

Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind B7-H2/ICOSLG, His & Avi, Human, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).

B7-H2/ICOSLG, His & Avi, Human

B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Z05057
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Product Introduction
Species Human
Protein Construction
B7-H2/ICOSLG (Asp19-Ser258)
Accession # O75144		 His Avi
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 29.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • B7-H2/ICOSLG, His & Avi, Human
    • B7-H2/ICOSLG, His & Avi, Human

    Immobilized B7-H2/ICOSLG, His & Avi, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA.

  • B7-H2/ICOSLG, His & Avi, Human
    • B7-H2/ICOSLG, His & Avi, Human

    The purity of B7-H2/ICOSLG, His & Avi, Human is greater than 90% as determined by SEC-HPLC.

  • B7-H2/ICOSLG, His & Avi, Human
    • B7-H2/ICOSLG, His & Avi, Human

    B7-H2/ICOSLG, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • B7-H2/ICOSLG, His & Avi, Human
    • B7-H2/ICOSLG, His & Avi, Human

    Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind B7-H2/ICOSLG, His & Avi, Human, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).


Background
Target Background B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Synonyms CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.