B7-H2/ICOSLG, His & Avi, Human
B7-H2/ICOSLG, His & Avi, Human

Immobilized B7-H2/ICOSLG, His & Avi, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA.

B7-H2/ICOSLG, His & Avi, Human

The purity of B7-H2/ICOSLG, His & Avi, Human is greater than 90% as determined by SEC-HPLC.

B7-H2/ICOSLG, His & Avi, Human

B7-H2/ICOSLG, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

B7-H2/ICOSLG, His & Avi, Human

Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind B7-H2/ICOSLG, His & Avi, Human, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).

B7-H2/ICOSLG, His & Avi, Human

B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Z05057
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Product Introduction
Species Human
Protein Construction
B7-H2/ICOSLG (Asp19-Ser258)
Accession # O75144		 His Avi
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized B7-H2/ICOSLG, His & Avi, Human at 1 μg/ml (100 μl/Well) on the plate can bind Human ICOS, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 29.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • B7-H2/ICOSLG, His & Avi, Human
    • B7-H2/ICOSLG, His & Avi, Human

    Immobilized B7-H2/ICOSLG, His & Avi, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA.

  • B7-H2/ICOSLG, His & Avi, Human
    • B7-H2/ICOSLG, His & Avi, Human

    The purity of B7-H2/ICOSLG, His & Avi, Human is greater than 90% as determined by SEC-HPLC.

  • B7-H2/ICOSLG, His & Avi, Human
    • B7-H2/ICOSLG, His & Avi, Human

    B7-H2/ICOSLG, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • B7-H2/ICOSLG, His & Avi, Human
    • B7-H2/ICOSLG, His & Avi, Human

    Human ICOS, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind B7-H2/ICOSLG, His & Avi, Human, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).


Background
Target Background B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Synonyms CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.