B7-2/CD86, His & Avi, Human
B7-2/CD86, His & Avi, Human

Immobilized B7-2/CD86, His & Avi, Human, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.

B7-2/CD86, His & Avi, Human

The purity of B7-2/CD86, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

B7-2/CD86, His & Avi, Human

B7-2/CD86, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

B7-2/CD86, His & Avi, Human

B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
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Product Introduction
Species Human
Protein Construction
B7-2/CD86 (Leu26-Pro247)
Accession # P42081-1		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 28.2 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • B7-2/CD86, His & Avi, Human
    • B7-2/CD86, His & Avi, Human

    Immobilized B7-2/CD86, His & Avi, Human, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.

  • B7-2/CD86, His & Avi, Human
    • B7-2/CD86, His & Avi, Human

    The purity of B7-2/CD86, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • B7-2/CD86, His & Avi, Human
    • B7-2/CD86, His & Avi, Human

    B7-2/CD86, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Synonyms CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.