APCDD1 hFc Chimera, Human
APCDD1 HFc Chimera, Human

Immobilized APCDD1 hFc Chimera, Human, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-APCDD1 Antibody, hFc Tag with the EC50 of 20.3 ng/ml determined by ELISA.

APCDD1 HFc Chimera, Human

APCDD1 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

APCDD1 hFc Chimera, Human

Adenomatosis polyposis coli downregulated 1 (APCDD1), a negative regulator of Wnt signaling, was examined to understand detailed mechanisms underlying Wnt signaling tooth development. In situ hybridization showed that Apcdd1 was expressed in the condensed mesenchyme at the bud stage, and in the inner enamel epithelium (IEE), including enamel knot (EK) at the cap stage. APCDD1 modulates the gene expression of Wnt- and EK-related signaling molecules at the cap stage of tooth development, and is involved in tooth cusp patterning by modulating the epithelial rearrangement in the IEE.
Z05029
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Product Introduction
Species Human
Protein Construction
APCDD1 (Leu27-His492)
Accession # Q8J025		 hFc
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized APCDD1 hFc Chimera, Human at 2μg/ml (100μl/well) on the plate can bind Biotinylated Anti­APCDD1 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 80.06 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 82-110 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • APCDD1 HFc Chimera, Human
    • APCDD1 HFc Chimera, Human

    Immobilized APCDD1 hFc Chimera, Human, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-APCDD1 Antibody, hFc Tag with the EC50 of 20.3 ng/ml determined by ELISA.

  • APCDD1 HFc Chimera, Human
    • APCDD1 HFc Chimera, Human

    APCDD1 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Adenomatosis polyposis coli downregulated 1 (APCDD1), a negative regulator of Wnt signaling, was examined to understand detailed mechanisms underlying Wnt signaling tooth development. In situ hybridization showed that Apcdd1 was expressed in the condensed mesenchyme at the bud stage, and in the inner enamel epithelium (IEE), including enamel knot (EK) at the cap stage. APCDD1 modulates the gene expression of Wnt- and EK-related signaling molecules at the cap stage of tooth development, and is involved in tooth cusp patterning by modulating the epithelial rearrangement in the IEE.
Synonyms Apcdd1; Drapc1; B7323; FP7019; HHS; HTS; HYPT1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.