AMIGO2, His, Human
AMIGO2, His, Human

The purity of AMIGO2, His, Human is greater than 95% as determined by SEC-HPLC.

AMIGO2, His, Human

AMIGO2, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

AMIGO2, His, Human

Bromodomain and extraterminal domain inhibitors (BETi) represent promising therapeutic agents for metastatic melanoma, yet their mechanism of action remains unclear. Here we interrogated the transcriptional effects of BETi and identified AMIGO2, a transmembrane molecule, as a BET target gene essential for melanoma cell survival. AMIGO2 is upregulated in melanoma cells and tissues compared to human melanocytes and nevi, and AMIGO2 silencing in melanoma cells induces G1/S arrest followed by apoptosis.
Z05017
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Product Introduction
Species Human
Protein Construction
AMIGO2 (Val40-Thr398)
Accession # Q86SJ2		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Expression System HEK293
Theoretical Molecular Weight 42 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • AMIGO2, His, Human
    • AMIGO2, His, Human

    The purity of AMIGO2, His, Human is greater than 95% as determined by SEC-HPLC.

  • AMIGO2, His, Human
    • AMIGO2, His, Human

    AMIGO2, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Bromodomain and extraterminal domain inhibitors (BETi) represent promising therapeutic agents for metastatic melanoma, yet their mechanism of action remains unclear. Here we interrogated the transcriptional effects of BETi and identified AMIGO2, a transmembrane molecule, as a BET target gene essential for melanoma cell survival. AMIGO2 is upregulated in melanoma cells and tissues compared to human melanocytes and nevi, and AMIGO2 silencing in melanoma cells induces G1/S arrest followed by apoptosis.
Synonyms Amphotein-induced protein 2; AMIGO-2; Alivin-1; DEGA; AMIGO2; ALI1; alivin 1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.