TNF alpha, His, Ferret
TNF Alpha, His, Ferret

Immobilized TNF alpha, His, Ferret, His Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA.

TNF Alpha, His, Ferret

The purity of TNF alpha, His, Ferret is greater than 95% as determined by SEC-HPLC.

TNF Alpha, His, Ferret

TNF alpha, His, Ferret on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

TNF alpha, His, Ferret

Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Z04960
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Product Introduction
Species Ferret
Protein Construction
TNF alpha (Val77-Leu233)
Accession # A3FBF1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System E.coli
Theoretical Molecular Weight 18.88 kDa
Apparent Molecular Weight The protein has a predicted MW of 18.88 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • TNF Alpha, His, Ferret
    • TNF Alpha, His, Ferret

    Immobilized TNF alpha, His, Ferret, His Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA.

  • TNF Alpha, His, Ferret
    • TNF Alpha, His, Ferret

    The purity of TNF alpha, His, Ferret is greater than 95% as determined by SEC-HPLC.

  • TNF Alpha, His, Ferret
    • TNF Alpha, His, Ferret

    TNF alpha, His, Ferret on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Synonyms APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.