Immobilized FcRn, His, Cynomolgus/Rhesus macaque, His Tag at 2 μg /ml (100 μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.

The purity of FcRn, His, Cynomolgus/Rhesus macaque is greater than 95% as determined by SEC-HPLC.

FcRn, His, Cynomolgus/Rhesus macaque on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

FcRn, His, Cynomolgus/Rhesus macaque, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.18 μM as determined in SPR assay (Biacore T200).

Cynomolgus Serum Albumin, His Tag immobilized on CM5 Chip can bind FcRn, His, Cynomolgus/Rhesus macaque, His Tag with an affinity constant of 0.13 μM as determined in SPR assay (Biacore T200).

FcRn, His, Cynomolgus/Rhesus macaque

The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.

Cat. No.	Z04951
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
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Price	¥72,752.00


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Product Introduction

Species

Cynomolgus/Rhesus macaque

Protein Construction

FcRn [Ala24-Ser297 (FCGRT) and Ile21-Met119 (B2M)] Accession # Q8SPV9 (FCGRT) & Q8SPW0 (B2M)	His
N-term	C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

31.5 kDa (FCGRT) and 11.6 kDa (B2M)

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT) and 13-15 kDa (B2M) based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

Immobilized FcRn, His, Cynomolgus/Rhesus macaque, His Tag at 2 μg /ml (100 μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.

The purity of FcRn, His, Cynomolgus/Rhesus macaque is greater than 95% as determined by SEC-HPLC.

FcRn, His, Cynomolgus/Rhesus macaque on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

FcRn, His, Cynomolgus/Rhesus macaque, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.18 μM as determined in SPR assay (Biacore T200).

Cynomolgus Serum Albumin, His Tag immobilized on CM5 Chip can bind FcRn, His, Cynomolgus/Rhesus macaque, His Tag with an affinity constant of 0.13 μM as determined in SPR assay (Biacore T200).

Background

Target Background

Synonyms

FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

FcRn, His, Cynomolgus/Rhesus macaque

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