DKK1 mFc Chimera, Cynomolgus/Rhesus macaque
DKK1 MFc Chimera, Cynomolgus/Rhesus Macaque

Immobilized DKK1 mFc Chimera, Cynomolgus/Rhesus macaque, mFc Tag at 1 μg /ml (100 μl/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA.

DKK1 MFc Chimera, Cynomolgus/Rhesus Macaque

The purity of DKK1 mFc Chimera, Cynomolgus/Rhesus macaque is greater than 95% as determined by SEC-HPLC.

DKK1 MFc Chimera, Cynomolgus/Rhesus Macaque

DKK1 mFc Chimera, Cynomolgus/Rhesus macaque on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

DKK1 mFc Chimera, Cynomolgus/Rhesus macaque

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Z04948
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Product Introduction
Species Cynomolgus/Rhesus macaque
Protein Construction
DKK1 (Thr32-His266)
Accession # XP_005565907.1 (Cynomolgus) / NP_001247454.1 (Rhesus macaque)		 mFc (IgG1)
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 51.42 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • DKK1 MFc Chimera, Cynomolgus/Rhesus Macaque
    • DKK1 MFc Chimera, Cynomolgus/Rhesus Macaque

    Immobilized DKK1 mFc Chimera, Cynomolgus/Rhesus macaque, mFc Tag at 1 μg /ml (100 μl/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA.

  • DKK1 MFc Chimera, Cynomolgus/Rhesus Macaque
    • DKK1 MFc Chimera, Cynomolgus/Rhesus Macaque

    The purity of DKK1 mFc Chimera, Cynomolgus/Rhesus macaque is greater than 95% as determined by SEC-HPLC.

  • DKK1 MFc Chimera, Cynomolgus/Rhesus Macaque
    • DKK1 MFc Chimera, Cynomolgus/Rhesus Macaque

    DKK1 mFc Chimera, Cynomolgus/Rhesus macaque on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Synonyms Dickkopf-1; Dkk1; SK

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.