TNF alpha, His, Cynomolgus
TNF Alpha, His, Cynomolgus

Immobilized TNF alpha, His, Cynomolgus, His Tag at 1 μg /ml (100 μl/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 1.5ng/ml determined by ELISA.

TNF Alpha, His, Cynomolgus

The purity of TNF alpha, His, Cynomolgus is greater than 95% as determined by SEC-HPLC.

TNF Alpha, His, Cynomolgus

TNF alpha, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

TNF Alpha, His, Cynomolgus

Cynomolgus TNFR1, His Tag immobilized on CM5 Chip can bind TNF alpha, His, Cynomolgus, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).

TNF alpha, His, Cynomolgus

Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Z04927
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Product Introduction
Species Cynomolgus
Protein Construction
His TNF alpha (Val77-Leu233)
Accession # P79337
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 18.35 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 19-25 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • TNF Alpha, His, Cynomolgus
    • TNF Alpha, His, Cynomolgus

    Immobilized TNF alpha, His, Cynomolgus, His Tag at 1 μg /ml (100 μl/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 1.5ng/ml determined by ELISA.

  • TNF Alpha, His, Cynomolgus
    • TNF Alpha, His, Cynomolgus

    The purity of TNF alpha, His, Cynomolgus is greater than 95% as determined by SEC-HPLC.

  • TNF Alpha, His, Cynomolgus
    • TNF Alpha, His, Cynomolgus

    TNF alpha, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • TNF Alpha, His, Cynomolgus
    • TNF Alpha, His, Cynomolgus

    Cynomolgus TNFR1, His Tag immobilized on CM5 Chip can bind TNF alpha, His, Cynomolgus, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).


Background
Target Background Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Synonyms APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.