IL-31, His, Cynomolgus
IL-31, His, Cynomolgus

Immobilized IL-31, His, Cynomolgus, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 0.58 μg/ml determined by ELISA.

IL-31, His, Cynomolgus

IL-31, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-31, His, Cynomolgus

IL-31, His, Cynomolgus, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-31 RA, His Tag with an affinity constant of 6.83 nM as determined in SPR assay (Biacore T200).

IL-31, His, Cynomolgus

Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells.
Z04841
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Product Introduction
Species Cynomolgus
Protein Construction
His IL-31 (Leu27-Thr163)
Accession # G7PJ78-1
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1 EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized IL-31, His, Cynomolgus at 5μg/ml (100μl/well) on the plate can bind Human IL­31 RA, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 16.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • IL-31, His, Cynomolgus
    • IL-31, His, Cynomolgus

    Immobilized IL-31, His, Cynomolgus, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 0.58 μg/ml determined by ELISA.

  • IL-31, His, Cynomolgus
    • IL-31, His, Cynomolgus

    IL-31, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • IL-31, His, Cynomolgus
    • IL-31, His, Cynomolgus

    IL-31, His, Cynomolgus, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-31 RA, His Tag with an affinity constant of 6.83 nM as determined in SPR assay (Biacore T200).


Background
Target Background Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells.
Synonyms Interleukin-31; IL-31;IL31

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.