IL-2[Biotin], His & Avi, Mouse
IL-2[Biotin], His & Avi, Mouse

Immobilized Mouse IL-2 R alpha, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for IL-2[Biotin], His & Avi, Mouse, His-Avi Tag with the EC50 of 0.16 μg/ml determined by ELISA.

IL-2[Biotin], His & Avi, Mouse

IL-2[Biotin], His & Avi, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-2[Biotin], His & Avi, Mouse

Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.
Z04668
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Product Introduction
Species Mouse
Protein Construction
Avi His IL-2 (Ala21-Gln169)
Accession # P04351-1
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per ug by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Mouse IL­2 R alpha, His Tag at 5μg/ml (100μl/well) on the plate can bind IL-2[Biotin], His & Avi, Mouse. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 20.1 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 27-30 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • IL-2[Biotin], His & Avi, Mouse
    • IL-2[Biotin], His & Avi, Mouse

    Immobilized Mouse IL-2 R alpha, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for IL-2[Biotin], His & Avi, Mouse, His-Avi Tag with the EC50 of 0.16 μg/ml determined by ELISA.

  • IL-2[Biotin], His & Avi, Mouse
    • IL-2[Biotin], His & Avi, Mouse

    IL-2[Biotin], His & Avi, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.
Synonyms IL2; T-cell growth factor; TCGF; Aldesleukin

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.