Immobilized Anti-VEGF165 Antibody, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for VEGF165[Biotin], His & Avi, Human, His Tag with the EC50 of 8.3 ng/ml determined by ELISA.

VEGF165[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

Immobilized Human VEGF R1, His Tag at 1μg/ml(100μl/well) on the plate. Dose response curve for VEGF165[Biotin], His & Avi, Human, His Tag with the EC50 of 10.5ng/ml determined by ELISA.

Immobilized Human VEGF R2, hFc Tag at 2μg/ml(100μl/well) on the plate. Dose response curve for VEGF165[Biotin], His & Avi, Human , His Tag with the EC50 of 12.6ng/ml determined by ELISA.

VEGF165[Biotin], His & Avi, Human, His Tag captured on CM5 Chip via streptavidin can bind Human VEGF R1, His Tag with an affinity constant of 1.36 pM as determined in SPR assay (Biacore T200).

VEGF165[Biotin], His & Avi, Human

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

Cat. No.	Z04643
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
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Price	¥123,118.00


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Product Introduction

Species

Human

Protein Construction

VEGF165 (Ala27-Arg191) Accession # P15692-4	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 90% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

22.2 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

Immobilized Anti-VEGF165 Antibody, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for VEGF165[Biotin], His & Avi, Human, His Tag with the EC50 of 8.3 ng/ml determined by ELISA.

VEGF165[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

Immobilized Human VEGF R1, His Tag at 1μg/ml(100μl/well) on the plate. Dose response curve for VEGF165[Biotin], His & Avi, Human, His Tag with the EC50 of 10.5ng/ml determined by ELISA.

Immobilized Human VEGF R2, hFc Tag at 2μg/ml(100μl/well) on the plate. Dose response curve for VEGF165[Biotin], His & Avi, Human , His Tag with the EC50 of 12.6ng/ml determined by ELISA.

VEGF165[Biotin], His & Avi, Human, His Tag captured on CM5 Chip via streptavidin can bind Human VEGF R1, His Tag with an affinity constant of 1.36 pM as determined in SPR assay (Biacore T200).

Background

Target Background	Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Synonyms	VEGF; VEGFA; VEGF165a; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

VEGF165[Biotin], His & Avi, Human

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