VEGF165[Biotin], His & Avi, Human
VEGF165[Biotin], His & Avi, Human

Immobilized Anti-VEGF165 Antibody, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for VEGF165[Biotin], His & Avi, Human, His Tag with the EC50 of 8.3 ng/ml determined by ELISA.

VEGF165[Biotin], His & Avi, Human

VEGF165[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

VEGF165[Biotin], His & Avi, Human

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Z04643
¥123,118.00

Ask us a question
Product Introduction
Species Human
Protein Construction
VEGF165 (Ala27-Arg191)
Accession # P15692-4		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Anti­VEGF165 Antibody at 1μg/ml (100μl/well) on the plate can bind VEGF165[Biotin], His & Avi, Human. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 22.2 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • VEGF165[Biotin], His & Avi, Human
    • VEGF165[Biotin], His & Avi, Human

    Immobilized Anti-VEGF165 Antibody, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for VEGF165[Biotin], His & Avi, Human, His Tag with the EC50 of 8.3 ng/ml determined by ELISA.

  • VEGF165[Biotin], His & Avi, Human
    • VEGF165[Biotin], His & Avi, Human

    VEGF165[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.


Background
Target Background Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Synonyms VEGF; VEGFA; VEGF165a; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.