MICB[Biotin], His & Avi, Human
MICB[Biotin], His & Avi, Human

Immobilized MICB[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.19 μg/ml determined by ELISA.

MICB[Biotin], His & Avi, Human

MICB[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

MICB[Biotin], His & Avi, Human

Immobilized Anti-MICB Antibody, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for MICB[Biotin], His & Avi, Human , His Tag with the EC50 of 0.43μg/ml determined by ELISA.

MICB[Biotin], His & Avi, Human

Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind MICB[Biotin], His & Avi, Human, His Tag with an affinity constant of 60.38 nM as determined in SPR assay (Biacore T200).

MICB[Biotin], His & Avi, Human

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Z04559
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Product Introduction
Species Human
Protein Construction
MICB (Ala23-Gly298)
Accession # Q29980		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. mmobilized MICB[Biotin], His & Avi, Human at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Human NKG2D, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 34.4 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • MICB[Biotin], His & Avi, Human
    • MICB[Biotin], His & Avi, Human

    Immobilized MICB[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.19 μg/ml determined by ELISA.

  • MICB[Biotin], His & Avi, Human
    • MICB[Biotin], His & Avi, Human

    MICB[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

  • MICB[Biotin], His & Avi, Human
    • MICB[Biotin], His & Avi, Human

    Immobilized Anti-MICB Antibody, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for MICB[Biotin], His & Avi, Human , His Tag with the EC50 of 0.43μg/ml determined by ELISA.

  • MICB[Biotin], His & Avi, Human
    • MICB[Biotin], His & Avi, Human

    Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind MICB[Biotin], His & Avi, Human, His Tag with an affinity constant of 60.38 nM as determined in SPR assay (Biacore T200).


Background
Target Background MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Synonyms MICB; MIC-B; PERB11.2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.