IL-13Ra1[Biotin], His & Avi, Human
IL-13Ra1[Biotin], His & Avi, Human

Immobilized IL-13Ra1[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 13.8 ng/ml determined by ELISA.

IL-13Ra1[Biotin], His & Avi, Human

The purity of IL-13Ra1[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

IL-13Ra1[Biotin], His & Avi, Human

IL-13Ra1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-13Ra1[Biotin], His & Avi, Human

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
Z04493
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Product Introduction
Species Human
Protein Construction
IL-13Ra1 (Ala27-Thr343)
Accession # P78552-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized IL-13Ra1[Biotin], His & Avi, Human at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Human IL­13, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 39.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55-75 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • IL-13Ra1[Biotin], His & Avi, Human
    • IL-13Ra1[Biotin], His & Avi, Human

    Immobilized IL-13Ra1[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 13.8 ng/ml determined by ELISA.

  • IL-13Ra1[Biotin], His & Avi, Human
    • IL-13Ra1[Biotin], His & Avi, Human

    The purity of IL-13Ra1[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • IL-13Ra1[Biotin], His & Avi, Human
    • IL-13Ra1[Biotin], His & Avi, Human

    IL-13Ra1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
Synonyms IL-13 R alpha 1; IL13R; IL13RA; CT19; IL13RA1; NR4; CD213A1; AI882074

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.