GARP&Latent TGF beta 1 Complex[Biotin], His & Avi, Human

GARP&Latent TGF beta 1 Complex[Biotin], His & Avi, Human

GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Z04467
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Product Introduction
Species Human
Protein Construction
GARP & Latent TGF beta 1 Complex [His20-Leu628 (GARP) and Leu30-Ser390 (Latent TGF beta 1)]
Accession # Q14392 (GARP) & P01137 (Latent TGF beta 1)		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Anti­GARP&Latent TGF beta 1 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate can bind GARP&Latent TGF beta 1 Complex[Biotin], His & Avi, Human. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 70.3 kDa (GARP) and 41.4 kDa (Latent TGF beta 1)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 73-78 kDa (GARP) and 13 kDa and 42-45 kDa (Latent TGF beta 1) based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Background
Target Background GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Synonyms LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.