FGL1[Biotin], His & Avi, Human
FGL1[Biotin], His & Avi, Human

Immobilized FGL1[Biotin], His & Avi, Human, His Tag at 5 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.19 μg/ml determined by ELISA.

FGL1[Biotin], His & Avi, Human

FGL1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..

FGL1[Biotin], His & Avi, Human

Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.
Z04463
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Product Introduction
Species Human
Protein Construction
Avi His FGL1 (Asp64-Asn305)
Accession # Q08830
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized FGL1[Biotin], His & Avi, Human at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Human LAG3, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 31.9 kDa
Apparent Molecular Weight The protein has a predicted MW of 31.9 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • FGL1[Biotin], His & Avi, Human
    • FGL1[Biotin], His & Avi, Human

    Immobilized FGL1[Biotin], His & Avi, Human, His Tag at 5 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human LAG3, hFc Tag with the EC50 of 0.19 μg/ml determined by ELISA.

  • FGL1[Biotin], His & Avi, Human
    • FGL1[Biotin], His & Avi, Human

    FGL1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..


Background
Target Background Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.
Synonyms FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.