FGF21 mFc Chimera[Biotin], Avi, Human
FGF21 MFc Chimera[Biotin], Avi, Human

Immobilized FGF21 mFc Chimera[Biotin], Avi, Human, mFc Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 96.8 ng/ml determined by ELISA.

FGF21 MFc Chimera[Biotin], Avi, Human

The purity of FGF21 mFc Chimera[Biotin], Avi, Human is greater than 95% as determined by SEC-HPLC.

FGF21 MFc Chimera[Biotin], Avi, Human

FGF21 mFc Chimera[Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

FGF21 mFc Chimera[Biotin], Avi, Human

Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
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Product Introduction
Species Human
Protein Construction
Avi mFc (IgG1) FGF21 (His29-Ser209)
Accession # Q9NSA1
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized FGF21 mFc Chimera[Biotin], Avi, Human at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Human Beta Klotho, His Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 46.9 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • FGF21 MFc Chimera[Biotin], Avi, Human
  • FGF21 MFc Chimera[Biotin], Avi, Human

    Immobilized FGF21 mFc Chimera[Biotin], Avi, Human, mFc Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 96.8 ng/ml determined by ELISA.

  • FGF21 MFc Chimera[Biotin], Avi, Human
  • FGF21 MFc Chimera[Biotin], Avi, Human

    The purity of FGF21 mFc Chimera[Biotin], Avi, Human is greater than 95% as determined by SEC-HPLC.

  • FGF21 MFc Chimera[Biotin], Avi, Human
  • FGF21 MFc Chimera[Biotin], Avi, Human

    FGF21 mFc Chimera[Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
Synonyms UNQ3115; PRO10196; FGF-21; FGF21

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.