Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human
Fc Gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human

Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5 μg/ml (100 μL/well) on the plate. Dose response curve for Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human, His Tag with the EC50 of 8.2 ng/ml determined by ELISA.

Fc Gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human

The purity of Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

Fc Gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human

Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Fc Gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human

Rituximab captured on CM5 Chip via Protein A can bind Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human , His-Avi Tag with an affinity constant of 0.539 μM as determined in SPR assay (Biacore T200).

Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human

The Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
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Product Introduction
Species Human
Protein Construction
Fc gamma RIIIA/CD16a (V176) [Gly17-Gln208 (V176)]
Accession # AAH17865		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 24.7 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • Fc Gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human
    • Fc Gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human

    Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5 μg/ml (100 μL/well) on the plate. Dose response curve for Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human, His Tag with the EC50 of 8.2 ng/ml determined by ELISA.

  • Fc Gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human
    • Fc Gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human

    The purity of Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • Fc Gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human
    • Fc Gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human

    Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • Fc Gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human
    • Fc Gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human

    Rituximab captured on CM5 Chip via Protein A can bind Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human , His-Avi Tag with an affinity constant of 0.539 μM as determined in SPR assay (Biacore T200).


Background
Target Background The Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Synonyms Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.