BTN3A1/CD277[Biotin], His & Avi, Human
BTN3A1/CD277[Biotin], His & Avi, Human

Immobilized BTN3A1/CD277[Biotin], His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 6.4 ng/ml determined by ELISA.

BTN3A1/CD277[Biotin], His & Avi, Human

The purity of BTN3A1/CD277[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

BTN3A1/CD277[Biotin], His & Avi, Human

BTN3A1/CD277[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

BTN3A1/CD277[Biotin], His & Avi, Human

The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
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Product Introduction
Species Human
Protein Construction
BTN3A1/CD277 (Gln30-Gly254)
Accession # O00481-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per ug by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized BTN3A1/CD277[Biotin], His & Avi, Human at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Anti­BTN3A1 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 27.1 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • BTN3A1/CD277[Biotin], His & Avi, Human
    • BTN3A1/CD277[Biotin], His & Avi, Human

    Immobilized BTN3A1/CD277[Biotin], His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 6.4 ng/ml determined by ELISA.

  • BTN3A1/CD277[Biotin], His & Avi, Human
    • BTN3A1/CD277[Biotin], His & Avi, Human

    The purity of BTN3A1/CD277[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • BTN3A1/CD277[Biotin], His & Avi, Human
    • BTN3A1/CD277[Biotin], His & Avi, Human

    BTN3A1/CD277[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
Synonyms CD277; BTN3A1; BTF5; BT3.1; BTN3.1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.