Immobilized Human BAFFR, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for BAFF/TNFSF13B/CD257[Biotin], His & Avi, Human, His Tag with the EC50 of 4.9 ng/ml determined by ELISA.

Immobilized Human BCMA, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for BAFF/TNFSF13B/CD257[Biotin], His & Avi, Human, His Avi Tag with the EC50 of 8.8 ng/ml determined by ELISA.

The purity of BAFF/TNFSF13B/CD257[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

BAFF/TNFSF13B/CD257[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

BAFF/TNFSF13B/CD257[Biotin], His & Avi, Human

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

Cat. No.	Z04413
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
Price	¥123,118.00


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Product Introduction

Species

Human

Protein Construction

Avi	His	BAFF/TNFSF13B/CD257 (Ala134-Leu285) Accession # Q9Y275-1
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by BisTris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Human BAFFR, His Tag at 0.5μg/ml (100μl/well) on the plate can bind BAFF/TNFSF13B/CD257[Biotin], His & Avi, Human. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

20.72 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 22-27 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples

The purity of BAFF/TNFSF13B/CD257[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

BAFF/TNFSF13B/CD257[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Background

Target Background	B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Synonyms	BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

BAFF/TNFSF13B/CD257[Biotin], His & Avi, Human

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