B7-H4[Biotin], His & Avi, Human
B7-H4[Biotin], His & Avi, Human

Immobilized B7-H4[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7.7 ng/ml determined by ELISA.

B7-H4[Biotin], His & Avi, Human

The purity of B7-H4[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

B7-H4[Biotin], His & Avi, Human

B7-H4[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

B7-H4[Biotin], His & Avi, Human

B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
Z04409
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Product Introduction
Species Human
Protein Construction
B7-H4 (Phe29-Ala258)
Accession # Q7Z7D3-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized B7-H4[Biotin], His & Avi, Human at 2μg/ml (100μl/Well) on streptavidin(5μg/ml) precoated plate can bind Anti­B7­H4 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 28.2 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • B7-H4[Biotin], His & Avi, Human
    • B7-H4[Biotin], His & Avi, Human

    Immobilized B7-H4[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7.7 ng/ml determined by ELISA.

  • B7-H4[Biotin], His & Avi, Human
    • B7-H4[Biotin], His & Avi, Human

    The purity of B7-H4[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • B7-H4[Biotin], His & Avi, Human
    • B7-H4[Biotin], His & Avi, Human

    B7-H4[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
Synonyms B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.