B7-1/CD80[Biotin], His & Avi, Human
B7-1/CD80[Biotin], His & Avi, Human

Immobilized B7-1/CD80[Biotin], His & Avi, Human, His Tag at 0.5 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.1 ng/ml determined by ELISA.

B7-1/CD80[Biotin], His & Avi, Human

The purity of B7-1/CD80[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

B7-1/CD80[Biotin], His & Avi, Human

B7-1/CD80[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

B7-1/CD80[Biotin], His & Avi, Human

Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
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Product Introduction
Species Human
Protein Construction
B7-1/CD80 (Val35-Asn242)
Accession # P33681-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized B7-1/CD80[Biotin], His & Avi, Human at 0.5μg/ml(100μl/Well) on streptavidin (5μg/ml) precoated plate can bind Human CTLA­4, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 26.8 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • B7-1/CD80[Biotin], His & Avi, Human
    • B7-1/CD80[Biotin], His & Avi, Human

    Immobilized B7-1/CD80[Biotin], His & Avi, Human, His Tag at 0.5 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.1 ng/ml determined by ELISA.

  • B7-1/CD80[Biotin], His & Avi, Human
    • B7-1/CD80[Biotin], His & Avi, Human

    The purity of B7-1/CD80[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • B7-1/CD80[Biotin], His & Avi, Human
    • B7-1/CD80[Biotin], His & Avi, Human

    B7-1/CD80[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Synonyms CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.