APRIL/TNFSF13 Trimer[Biotin], His & Avi & Flag, Human

APRIL/TNFSF13 Trimer[Biotin], His & Avi & Flag, Human

The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
Z04401
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Product Introduction
Species Human
Protein Construction
Avi His APRIL/TNFSF13 Trimer (Lys112-Leu250)
Accession # O75888-1
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
> 90% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Human BCMA, hFc Tag at 0.5μg/ml (100μl/well) on the plate can bind APRIL/TNFSF13 Trimer[Biotin], His & Avi & Flag, Human. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 52.1 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Background
Target Background The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.
Synonyms CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2; 2310026N09Rik

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.