GARP&Latent TGF beta 1 Complex[Biotin], His & Avi, Cynomolgus
GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Cynomolgus

Immobilized GARP&Latent TGF beta 1 Complex[Biotin], His & Avi, Cynomolgus, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 6.6 ng/ml determined by ELISA.

GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Cynomolgus

The purity of GARP&Latent TGF beta 1 Complex[Biotin], His & Avi, Cynomolgus is greater than 90% as determined by SEC-HPLC.

GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Cynomolgus

GARP&Latent TGF beta 1 Complex[Biotin], His & Avi, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

GARP&Latent TGF beta 1 Complex[Biotin], His & Avi, Cynomolgus

GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Z04365
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Product Introduction
Species Cynomolgus
Protein Construction
GARP & Latent TGF beta 1 Complex [Ala18-Leu628 (GARP) & Leu30-Ser390 (Latent TGF beta 1)]
Accession # A0A2K5X2X9 (GARP) & A0A2K5TJB2 (Latent TGF beta 1)		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 70.5 kDa (GARP)&41.3 kDa (Latent TGF Beta 1)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF Beta) based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Cynomolgus
    • GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Cynomolgus

    Immobilized GARP&Latent TGF beta 1 Complex[Biotin], His & Avi, Cynomolgus, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 6.6 ng/ml determined by ELISA.

  • GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Cynomolgus
    • GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Cynomolgus

    The purity of GARP&Latent TGF beta 1 Complex[Biotin], His & Avi, Cynomolgus is greater than 90% as determined by SEC-HPLC.

  • GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Cynomolgus
    • GARP&Latent TGF Beta 1 Complex[Biotin], His & Avi, Cynomolgus

    GARP&Latent TGF beta 1 Complex[Biotin], His & Avi, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Synonyms LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.