CD28, His, Rat
CD28, His, Rat

CD28, His, Rat on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD28, His, Rat

Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind CD28, His, Rat, His Tag with an affinity constant of 42.64 μM as determined in SPR assay (Biacore T200).

CD28, His, Rat

CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Z04350
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Product Introduction
Species Rat
Protein Construction
CD28 (Asn20-Leu150)
Accession # P31042		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Human B7­1, hFc Tag captured on CM5 Chip via Protein A can bind CD28, His, Rat in SPR assay (Biacore T200).Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 16.66 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • CD28, His, Rat
    • CD28, His, Rat

    CD28, His, Rat on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • CD28, His, Rat
    • CD28, His, Rat

    Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind CD28, His, Rat, His Tag with an affinity constant of 42.64 μM as determined in SPR assay (Biacore T200).


Background
Target Background CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Synonyms CD28 antigen (Tp44); CD28 molecule; CD28

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.