NKG2D/CD314, His, Mouse
NKG2D/CD314, His, Mouse

Immobilized NKG2D/CD314, His, Mouse, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 70.5 ng/ml determined by ELISA.

NKG2D/CD314, His, Mouse

The purity of NKG2D/CD314, His, Mouse is greater than 95% as determined by SEC-HPLC.

NKG2D/CD314, His, Mouse

NKG2D/CD314, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

NKG2D/CD314, His, Mouse

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Z04344
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Product Introduction
Species Mouse
Protein Construction
His NKG2D/CD314 (Phe90-Val232)
Accession # O54709-1
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized NKG2D/CD314, His, Mouse at 5μg/ml (100μl/well) on the plate can bind Human MICA, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 17.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 24-35kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris, 250mM NaCl (pH 8.0).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • NKG2D/CD314, His, Mouse
    • NKG2D/CD314, His, Mouse

    Immobilized NKG2D/CD314, His, Mouse, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 70.5 ng/ml determined by ELISA.

  • NKG2D/CD314, His, Mouse
    • NKG2D/CD314, His, Mouse

    The purity of NKG2D/CD314, His, Mouse is greater than 95% as determined by SEC-HPLC.

  • NKG2D/CD314, His, Mouse
    • NKG2D/CD314, His, Mouse

    NKG2D/CD314, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Synonyms CD314; D12S2489E; KLR; NKG2-D; NKG2D

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.