IL-2 R alpha/CD25, His, Mouse
IL-2 R Alpha/CD25, His, Mouse

Immobilized IL-2 R alpha/CD25, His, Mouse, His Tag at 5 μg/ml (100 μL/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.17 μg/ml determined by ELISA.

IL-2 R Alpha/CD25, His, Mouse

The purity of IL-2 R alpha/CD25, His, Mouse is greater than 95% as determined by SEC-HPLC.

IL-2 R Alpha/CD25, His, Mouse

IL-2 R alpha/CD25, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-2 R Alpha/CD25, His, Mouse

IL-2 R alpha/CD25, His, Mouse, His Tag immobilized on CM5 Chip can bind Mouse IL-2, His Tag with an affinity constant of 30.49 nM as determined in SPR assay (Biacore T200).

IL-2 R alpha/CD25, His, Mouse

Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Z04342
¥69,021.00

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Product Introduction
Species Mouse
Protein Construction
IL-2 R alpha/CD25 (Glu22-Lys236)
Accession # P01590		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized IL-2 R alpha/CD25, His, Mouse at 5μg/ml (100μl/well) on the plate can bind Biotinylated Mouse IL­2, His Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 25.76 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • IL-2 R Alpha/CD25, His, Mouse
    • IL-2 R Alpha/CD25, His, Mouse

    Immobilized IL-2 R alpha/CD25, His, Mouse, His Tag at 5 μg/ml (100 μL/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.17 μg/ml determined by ELISA.

  • IL-2 R Alpha/CD25, His, Mouse
    • IL-2 R Alpha/CD25, His, Mouse

    The purity of IL-2 R alpha/CD25, His, Mouse is greater than 95% as determined by SEC-HPLC.

  • IL-2 R Alpha/CD25, His, Mouse
    • IL-2 R Alpha/CD25, His, Mouse

    IL-2 R alpha/CD25, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • IL-2 R Alpha/CD25, His, Mouse
    • IL-2 R Alpha/CD25, His, Mouse

    IL-2 R alpha/CD25, His, Mouse, His Tag immobilized on CM5 Chip can bind Mouse IL-2, His Tag with an affinity constant of 30.49 nM as determined in SPR assay (Biacore T200).


Background
Target Background Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Synonyms CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.