Fc gamma RIIB/CD32b, His, Mouse
Fc Gamma RIIB/CD32b, His, Mouse

The purity of Fc gamma RIIB/CD32b, His, Mouse is greater than 95% as determined by SEC-HPLC.

Fc Gamma RIIB/CD32b, His, Mouse

Fc gamma RIIB/CD32b, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Fc Gamma RIIB/CD32b, His, Mouse

OKT3 captured on CM5 Chip via Protein A can bind Fc gamma RIIB/CD32b, His, Mouse, His Tag with an affinity constant of 0.280 μM as determined in SPR assay (Biacore T200).

Fc gamma RIIB/CD32b, His, Mouse

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
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Product Introduction
Species Mouse
Protein Construction
Fc gamma RIIB/CD32b (Thr40-Arg217)
Accession # NP_001070657.1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per ug by the LAL method.
Biological Activity OKT3 captured on CM5 Chip via Protein A can bind Fc gamma RIIB/CD32b, His, Mouse in SPR assay (Biacore T200). Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 21.4 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • Fc Gamma RIIB/CD32b, His, Mouse
    • Fc Gamma RIIB/CD32b, His, Mouse

    The purity of Fc gamma RIIB/CD32b, His, Mouse is greater than 95% as determined by SEC-HPLC.

  • Fc Gamma RIIB/CD32b, His, Mouse
    • Fc Gamma RIIB/CD32b, His, Mouse

    Fc gamma RIIB/CD32b, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • Fc Gamma RIIB/CD32b, His, Mouse
    • Fc Gamma RIIB/CD32b, His, Mouse

    OKT3 captured on CM5 Chip via Protein A can bind Fc gamma RIIB/CD32b, His, Mouse, His Tag with an affinity constant of 0.280 μM as determined in SPR assay (Biacore T200).


Background
Target Background The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
Synonyms Fc gamma RII; Fc gamma RIIB; CD32; CD32b; CDw32; FCG2; IGFR2; CD32A; FCG2 ; FcGR; FCGR2; FCGR21; FCGR2A; FcγRIIB; Fc γRIIB; Fcγ RIIB; Fc γ RIIB

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.