CD28 hFc Chimera, Human/Cynomolgus/Rhesus macaque
CD28 HFc Chimera, Human/Cynomolgus/Rhesus Macaque

Immobilized CD28 hFc Chimera, Human/Cynomolgus/Rhesus macaque, hFc Tag at 0.5 μg/ml (100 μL/well) on the plate. Dose response curve for Biotinylated Anti-CD28 Antibody, hFc Tag with the EC50 of 43.5 ng/ml determined by ELISA.

CD28 HFc Chimera, Human/Cynomolgus/Rhesus Macaque

The purity of CD28 hFc Chimera, Human/Cynomolgus/Rhesus macaque was greater than 95% as determined by SEC-HPLC.

CD28 HFc Chimera, Human/Cynomolgus/Rhesus Macaque

CD28 hFc Chimera, Human/Cynomolgus/Rhesus macaque on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD28 hFc Chimera, Human/Cynomolgus/Rhesus macaque

CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
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Product Introduction
Species Human/Cynomolgus/Rhesus macaque
Protein Construction
hFc CD28 (Asn19-Pro152)
Accession # P10747-1
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD28 hFc Chimera, Human/Cynomolgus/Rhesus macaque at 0.5μg/ml (100μl/Well) on the plate can bind Biotinylated Anti­CD28 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 42.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • CD28 HFc Chimera, Human/Cynomolgus/Rhesus Macaque
    • CD28 HFc Chimera, Human/Cynomolgus/Rhesus Macaque

    Immobilized CD28 hFc Chimera, Human/Cynomolgus/Rhesus macaque, hFc Tag at 0.5 μg/ml (100 μL/well) on the plate. Dose response curve for Biotinylated Anti-CD28 Antibody, hFc Tag with the EC50 of 43.5 ng/ml determined by ELISA.

  • CD28 HFc Chimera, Human/Cynomolgus/Rhesus Macaque
    • CD28 HFc Chimera, Human/Cynomolgus/Rhesus Macaque

    The purity of CD28 hFc Chimera, Human/Cynomolgus/Rhesus macaque was greater than 95% as determined by SEC-HPLC.

  • CD28 HFc Chimera, Human/Cynomolgus/Rhesus Macaque
    • CD28 HFc Chimera, Human/Cynomolgus/Rhesus Macaque

    CD28 hFc Chimera, Human/Cynomolgus/Rhesus macaque on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Synonyms CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.