Siglec-3/CD33, His & Avi, Human
Siglec-3/CD33, His & Avi, Human

Immobilized Siglec-3/CD33, His & Avi, Human, His Tag at 1 μg/ml (100 μL/well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.2 ng/ml determined by ELISA.

Siglec-3/CD33, His & Avi, Human

The purity of Siglec-3/CD33, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

Siglec-3/CD33, His & Avi, Human

Siglec-3/CD33, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Siglec-3/CD33, His & Avi, Human

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
Z04280
¥55,963.00

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Product Introduction
Species Human
Protein Construction
Siglec-3/CD33 (Asp18-His259)
Accession # P20138-1		 His Avi
N-term C-term
Purity > 95% as determined by Tris­Bis PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Siglec-3/CD33, His & Avi, Human at 1 μg/ml (100 μl/Well) on the plate can bind Anti­-Siglec­3-Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 29.6 kDa
Formulation Lyophilized from 0.22μm filtered solution in 20 mM MES, 500 mM NaCl, 200 mM L-Arginine (pH 5.0).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • Siglec-3/CD33, His & Avi, Human
    • Siglec-3/CD33, His & Avi, Human

    Immobilized Siglec-3/CD33, His & Avi, Human, His Tag at 1 μg/ml (100 μL/well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.2 ng/ml determined by ELISA.

  • Siglec-3/CD33, His & Avi, Human
    • Siglec-3/CD33, His & Avi, Human

    The purity of Siglec-3/CD33, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • Siglec-3/CD33, His & Avi, Human
    • Siglec-3/CD33, His & Avi, Human

    Siglec-3/CD33, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
Synonyms CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.