Immobilized NKG2D/CD314, His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 3.3 ng/ml determined by ELISA.

NKG2D/CD314, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

NKG2D/CD314 hFc Chimera, Flag, Human, His Tag at 5 μg/ml (100 μl/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.

NKG2D/CD314 hFc Chimera, Flag, Human, His Tag at 2 μg/ml(100 μl/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.

Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind NKG2D/CD314 hFc Chimera, Flag, Human , His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).

NKG2D/CD314, His & Avi, Human

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.

Cat. No.	Z04279
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
Price	¥63,425.00


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Product Introduction

Species

Human

Protein Construction

His	Avi	NKG2D/CD314 (Phe78-Val216) Accession # P26718
N-term		C-term

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized NKG2D/CD314, His & Avi, Human at 1μg/ml (100μl/well) on the plate can bind AntiNKG2D Antibody, hFc Tag. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

19.0 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 36-38 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples

Immobilized NKG2D/CD314, His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 3.3 ng/ml determined by ELISA.

NKG2D/CD314, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

NKG2D/CD314 hFc Chimera, Flag, Human, His Tag at 5 μg/ml (100 μl/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.

NKG2D/CD314 hFc Chimera, Flag, Human, His Tag at 2 μg/ml(100 μl/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.

Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind NKG2D/CD314 hFc Chimera, Flag, Human , His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).

Background

Target Background	NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Synonyms	CD314; D12S2489E; KLR; NKG2-D; NKG2D

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

NKG2D/CD314, His & Avi, Human

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