NKG2D/CD314 hFc Chimera, Flag, Human
NKG2D/CD314 HFc Chimera, Flag, Human

Immobilized Human MICA, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for NKG2D/CD314 hFc Chimera, Flag, Human, hFc Tag with the EC50 of 0.94 μg/ml determined by ELISA.

NKG2D/CD314 HFc Chimera, Flag, Human

The purity of NKG2D/CD314 hFc Chimera, Flag, Human is greater than 95% as determined by SEC-HPLC.

NKG2D/CD314 HFc Chimera, Flag, Human

NKG2D/CD314 hFc Chimera, Flag, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

NKG2D/CD314 HFc Chimera, Flag, Human

Serial dilutions of Anti- NKG2D Antibody were added into Biotinylated Human ULBP-2, His Tag : NKG2D/CD314 hFc Chimera, Flag, Human, hFc Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 45.2 ng/ml.

NKG2D/CD314 hFc Chimera, Flag, Human

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Z04278
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Product Introduction
Species Human
Protein Construction
hFc Flag NKG2D/CD314 (Phe78-Val216)
Accession # P26718
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Human MICA, His Tag at 5μg/ml (100μl/Well) on the plate can bind Human NKG2D/CD314 hFc Chimera, Flag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 43.4 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • NKG2D/CD314 HFc Chimera, Flag, Human
    • NKG2D/CD314 HFc Chimera, Flag, Human

    Immobilized Human MICA, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for NKG2D/CD314 hFc Chimera, Flag, Human, hFc Tag with the EC50 of 0.94 μg/ml determined by ELISA.

  • NKG2D/CD314 HFc Chimera, Flag, Human
    • NKG2D/CD314 HFc Chimera, Flag, Human

    The purity of NKG2D/CD314 hFc Chimera, Flag, Human is greater than 95% as determined by SEC-HPLC.

  • NKG2D/CD314 HFc Chimera, Flag, Human
    • NKG2D/CD314 HFc Chimera, Flag, Human

    NKG2D/CD314 hFc Chimera, Flag, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • NKG2D/CD314 HFc Chimera, Flag, Human
    • NKG2D/CD314 HFc Chimera, Flag, Human

    Serial dilutions of Anti- NKG2D Antibody were added into Biotinylated Human ULBP-2, His Tag : NKG2D/CD314 hFc Chimera, Flag, Human, hFc Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 45.2 ng/ml.


Background
Target Background NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Synonyms CD314; D12S2489E; KLR; NKG2-D; NKG2D

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.