CD8 alpha&beta Heterodimer, His & Flag, Human

CD8 alpha&beta Heterodimer, His & Flag, Human

CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+ peripheral T cells bearing TCR.
Z04227
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Product Introduction
Species Human
Protein Construction
CD8 alpha & beta Heterodimer [Ser22-Asp182 (CD8 alpha) acidic tail and Leu22-Pro170 (CD8 beta) basic tail]
Accession # P01732-1 (CD8 alpha) & P10966-1 (CD8 beta)		 His Flag
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD8 alpha&beta Heterodimer, His & Flag, Human at 2μg/ml (100μl/well) on the plate can bind Anti-CD8 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 23.54 kDa (CD8 alpha) and 22.62 kDa (CD8 beta)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 26-28 kDa (CD8 alpha) and 28-32 kDa (CD8 beta) on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Background
Target Background CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+ peripheral T cells bearing TCR.
Synonyms CD8 alphaβ CD8A&CD8B

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.