CD30 Ligand/TNFSF8, His, Human
CD30 Ligand/TNFSF8, His, Human

Immobilized CD30 Ligand/TNFSF8, His, Human, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8 ng/ml determined by ELISA.

CD30 Ligand/TNFSF8, His, Human

The purity of CD30 Ligand/TNFSF8, His, Human is greater than 95% as determined by SEC-HPLC.

CD30 Ligand/TNFSF8, His, Human

CD30 Ligand/TNFSF8, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD30 Ligand/TNFSF8, His, Human

CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Z04185
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Product Introduction
Species Human
Protein Construction
His CD30 Ligand/TNFSF8 (Gln63-Asp234)
Accession # P32971
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD30 Ligand/TNFSF8, His, Human at 5μg/ml (100μl/well) on the plate can bind Human CD30, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 20.7 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 30-50 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • CD30 Ligand/TNFSF8, His, Human
    • CD30 Ligand/TNFSF8, His, Human

    Immobilized CD30 Ligand/TNFSF8, His, Human, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8 ng/ml determined by ELISA.

  • CD30 Ligand/TNFSF8, His, Human
    • CD30 Ligand/TNFSF8, His, Human

    The purity of CD30 Ligand/TNFSF8, His, Human is greater than 95% as determined by SEC-HPLC.

  • CD30 Ligand/TNFSF8, His, Human
    • CD30 Ligand/TNFSF8, His, Human

    CD30 Ligand/TNFSF8, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Synonyms CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.