NKG2D/CD314[Biotin], His & Avi, Human
NKG2D/CD314[Biotin], His & Avi, Human

Immobilized NKG2D/CD314[Biotin], His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human MICA, hFc Tag with the EC50 of 2.7 ng/ml determined by ELISA.

NKG2D/CD314[Biotin], His & Avi, Human

The purity of NKG2D/CD314[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

NKG2D/CD314[Biotin], His & Avi, Human

NKG2D/CD314[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

NKG2D/CD314[Biotin], His & Avi, Human

Immobilized Anti-NKG2D Antibody at 2μg/ml(100μI/well) on the plate. Dose response curve for NKG2D/CD314[Biotin], His & Avi, Human, His Tag with the EC50 of 0.36ng/ml determined by ELISA.

NKG2D/CD314[Biotin], His & Avi, Human

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
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Product Introduction
Species Human
Protein Construction
His Avi NKG2D/CD314 (Phe78-Val216)
Accession # P26718
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized NKG2D/CD314[Biotin], His & Avi, Human at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Human MICA, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 19.0 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 36-40 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • NKG2D/CD314[Biotin], His & Avi, Human
    • NKG2D/CD314[Biotin], His & Avi, Human

    Immobilized NKG2D/CD314[Biotin], His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human MICA, hFc Tag with the EC50 of 2.7 ng/ml determined by ELISA.

  • NKG2D/CD314[Biotin], His & Avi, Human
    • NKG2D/CD314[Biotin], His & Avi, Human

    The purity of NKG2D/CD314[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • NKG2D/CD314[Biotin], His & Avi, Human
    • NKG2D/CD314[Biotin], His & Avi, Human

    NKG2D/CD314[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • NKG2D/CD314[Biotin], His & Avi, Human
    • NKG2D/CD314[Biotin], His & Avi, Human

    Immobilized Anti-NKG2D Antibody at 2μg/ml(100μI/well) on the plate. Dose response curve for NKG2D/CD314[Biotin], His & Avi, Human, His Tag with the EC50 of 0.36ng/ml determined by ELISA.


Background
Target Background NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Synonyms CD314; D12S2489E; KLR; NKG2-D; NKG2D

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.