The purity of Fc gamma RIIB/CD32b[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.
Fc gamma RIIB/CD32b[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Fc gamma RIIB/CD32b[Biotin], His & Avi, Human, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 5.87 μM as determined in SPR assay (Biacore T200) (QC Test).
Rituximab captured on CM5 Chip via Protein A can bindFc gamma RIIB/CD32b[Biotin], His & Avi, Human, His-Avi Tag with an affinity constant of 3.97 μM as determined in SPR assay (Biacore T200).
Fc gamma RIIB/CD32b[Biotin], His & Avi, Human
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
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