CD47[Biotin], His & Avi, Human
CD47[Biotin], His & Avi, Human

Immobilized CD47[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.

CD47[Biotin], His & Avi, Human

The purity of CD47[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

CD47[Biotin], His & Avi, Human

CD47[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD47[Biotin], His & Avi, Human

CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Z04070
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Product Introduction
Species Human
Protein Construction
CD47 (GIn19-Pro139)
Accession # Q08722-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 18.38 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • CD47[Biotin], His & Avi, Human
    • CD47[Biotin], His & Avi, Human

    Immobilized CD47[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.

  • CD47[Biotin], His & Avi, Human
    • CD47[Biotin], His & Avi, Human

    The purity of CD47[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • CD47[Biotin], His & Avi, Human
    • CD47[Biotin], His & Avi, Human

    CD47[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Synonyms CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.