CD300A[Biotin], Avi, Human
CD300A[Biotin], Avi, Human

Immobilized CD300A[Biotin], Avi, Human, Avi Tag at 1 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 1.3 ng/ml determined by ELISA.

CD300A[Biotin], Avi, Human

The purity of CD300A[Biotin], Avi, Human is greater than 95% as determined by SEC-HPLC.

CD300A[Biotin], Avi, Human

CD300A[Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD300A[Biotin], Avi, Human

CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation.
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Product Introduction
Species Human
Protein Construction
CD300A (Leu18-Pro180)
Accession # Q9UGN4-1		 Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD300A[Biotin], Avi, Human at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Anti­CD300A Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 19.46 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • CD300A[Biotin], Avi, Human
    • CD300A[Biotin], Avi, Human

    Immobilized CD300A[Biotin], Avi, Human, Avi Tag at 1 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 1.3 ng/ml determined by ELISA.

  • CD300A[Biotin], Avi, Human
    • CD300A[Biotin], Avi, Human

    The purity of CD300A[Biotin], Avi, Human is greater than 95% as determined by SEC-HPLC.

  • CD300A[Biotin], Avi, Human
    • CD300A[Biotin], Avi, Human

    CD300A[Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation.
Synonyms IGSF12; IRp60; CLM-8; IRC1; CD300a; CLM8; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.