CD24 mFc Chimera[Biotin], Human
CD24 MFc Chimera[Biotin], Human

Immobilized Anti-CD24 Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for CD24 mFc Chimera[Biotin], Human, mFc Tag with the EC50 of 0.31 μg/ml determined by ELISA.

CD24 MFc Chimera[Biotin], Human

The purity of CD24 mFc Chimera[Biotin], Human is greater than 95% as determined by SEC-HPLC.

CD24 MFc Chimera[Biotin], Human

CD24 mFc Chimera[Biotin], Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

CD24 mFc Chimera[Biotin], Human

CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.
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Product Introduction
Species Human
Protein Construction
CD24 (Ser27-Gly59)
Accession # P25063-1		 mFc (IgG1)
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Anti-CD24 Antibody at 2μg/ml (100μl/well) on the plate can bind CD24 mFc Chimera[Biotin], Human. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 29.5 kD
Apparent Molecular Weight Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • CD24 MFc Chimera[Biotin], Human
    • CD24 MFc Chimera[Biotin], Human

    Immobilized Anti-CD24 Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for CD24 mFc Chimera[Biotin], Human, mFc Tag with the EC50 of 0.31 μg/ml determined by ELISA.

  • CD24 MFc Chimera[Biotin], Human
    • CD24 MFc Chimera[Biotin], Human

    The purity of CD24 mFc Chimera[Biotin], Human is greater than 95% as determined by SEC-HPLC.

  • CD24 MFc Chimera[Biotin], Human
    • CD24 MFc Chimera[Biotin], Human

    CD24 mFc Chimera[Biotin], Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.


Background
Target Background CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.
Synonyms CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.