CD24[Biotin], His & Avi, Human
CD24[Biotin], His & Avi, Human

Immobilized CD24[Biotin], His & Avi, Human, His Tag at 1 μg/ml(100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 5.5 ng/ml determined by ELISA.

CD24[Biotin], His & Avi, Human

The purity of CD24[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

CD24[Biotin], His & Avi, Human

CD24[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD24[Biotin], His & Avi, Human

CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.
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Product Introduction
Species Human
Protein Construction
CD24 (Ser27-Gly59)
Accession # P25063-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD24[Biotin], His & Avi, Human at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Anti-CD24 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 6.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • CD24[Biotin], His & Avi, Human
    • CD24[Biotin], His & Avi, Human

    Immobilized CD24[Biotin], His & Avi, Human, His Tag at 1 μg/ml(100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 5.5 ng/ml determined by ELISA.

  • CD24[Biotin], His & Avi, Human
    • CD24[Biotin], His & Avi, Human

    The purity of CD24[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • CD24[Biotin], His & Avi, Human
    • CD24[Biotin], His & Avi, Human

    CD24[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.
Synonyms CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.