CD117[Biotin], His & Avi, Human
CD117[Biotin], His & Avi, Human

Immobilized Human SCF, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for CD117[Biotin], His & Avi, Human, His Tag with the EC50 of 6.8 μg/ml determined by ELISA.

CD117[Biotin], His & Avi, Human

The purity of CD117[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

CD117[Biotin], His & Avi, Human

CD117[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD117[Biotin], His & Avi, Human

The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.
Z04051
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Product Introduction
Species Human
Protein Construction
CD117 (Gln26-Pro520)
Accession # P10721-2		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 58.60 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • CD117[Biotin], His & Avi, Human
    • CD117[Biotin], His & Avi, Human

    Immobilized Human SCF, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for CD117[Biotin], His & Avi, Human, His Tag with the EC50 of 6.8 μg/ml determined by ELISA.

  • CD117[Biotin], His & Avi, Human
    • CD117[Biotin], His & Avi, Human

    The purity of CD117[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • CD117[Biotin], His & Avi, Human
    • CD117[Biotin], His & Avi, Human

    CD117[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.
Synonyms SCFR; PBT; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.