CD4/LEU3 [Biotin], His & Avi, Human
CD4/LEU3 [Biotin], His & Avi, Human

Immobilized Anti-CD4 Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for CD4/LEU3[Biotin], His & Avi, Human, His Tag with the EC50 of 8.1 ng/ml determined by ELISA.

CD4/LEU3 [Biotin], His & Avi, Human

The purity of CD4/LEU3[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

CD4/LEU3 [Biotin], His & Avi, Human

CD4/LEU3[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD4/LEU3 [Biotin], His & Avi, Human

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Z04015
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Product Introduction
Species Human
Protein Construction
CD4/LEU3 (Lys26-Trp390)
Accession # P01730		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Anti-CD4 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate can bind CD4/LEU3 [Biotin], His & Avi, Human. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 43.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS，(pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • CD4/LEU3 [Biotin], His & Avi, Human
    • CD4/LEU3 [Biotin], His & Avi, Human

    Immobilized Anti-CD4 Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for CD4/LEU3[Biotin], His & Avi, Human, His Tag with the EC50 of 8.1 ng/ml determined by ELISA.

  • CD4/LEU3 [Biotin], His & Avi, Human
    • CD4/LEU3 [Biotin], His & Avi, Human

    The purity of CD4/LEU3[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • CD4/LEU3 [Biotin], His & Avi, Human
    • CD4/LEU3 [Biotin], His & Avi, Human

    CD4/LEU3[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Synonyms CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.